Florence Mills House is a house at 220 West 135th Street in Harlem, Manhattan, New York City. The house was originally believed to be the residence of Florence Mills, a leading African-American actress and entertainer during the 1920s. She lived at this address, or a similar address a few blocks away, during her most productive years from 1910 to 1927. The 220 West 135th Street building that existed in 1927 no longer stands and has been replaced. The site was designated a National Historic Landmark in 1976.